Repression of flagellar genes in exponential phase by CsgD and CpxR, two crucial modulators of Escherichia coli biofilm formation.

Details

Serval ID
serval:BIB_5C0EBC04840B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Repression of flagellar genes in exponential phase by CsgD and CpxR, two crucial modulators of Escherichia coli biofilm formation.
Journal
Journal of Bacteriology
Author(s)
Dudin O., Geiselmann J., Ogasawara H., Ishihama A., Lacour S.
ISSN
1098-5530 (Electronic)
ISSN-L
0021-9193
Publication state
Published
Issued date
2014
Volume
196
Number
3
Pages
707-715
Language
english
Abstract
Escherichia coli adapts its lifestyle to the variations of environmental growth conditions, swapping between swimming motility or biofilm formation. The stationary-phase sigma factor RpoS is an important regulator of this switch, since it stimulates adhesion and represses flagellar biosynthesis. By measuring the dynamics of gene expression, we show that RpoS inhibits the transcription of the flagellar sigma factor, FliA, in exponential growth phase. RpoS also partially controls the expression of CsgD and CpxR, two transcription factors important for bacterial adhesion. We demonstrate that these two regulators repress the transcription of fliA, flgM, and tar and that this regulation is dependent on the growth medium. CsgD binds to the flgM and fliA promoters around their -10 promoter element, strongly suggesting direct repression. We show that CsgD and CpxR also affect the expression of other known modulators of cell motility. We propose an updated structure of the regulatory network controlling the choice between adhesion and motility.
Keywords
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Biofilms/growth & development, Cyclic AMP/genetics, Cyclic AMP/metabolism, Escherichia coli/physiology, Escherichia coli Proteins/genetics, Escherichia coli Proteins/metabolism, Flagella/genetics, Flagella/physiology, Gene Expression Regulation, Bacterial/physiology, Sigma Factor/genetics, Sigma Factor/metabolism, Trans-Activators/genetics, Trans-Activators/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
21/04/2014 16:50
Last modification date
20/08/2019 15:14
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