Immobilization of proteinases on chitosan

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State: Public
Version: author
Serval ID
serval:BIB_588DDDB20CEB
Type
Article: article from journal or magazin.
Collection
Publications
Title
Immobilization of proteinases on chitosan
Journal
Biotechnology Letters
Author(s)
Leuba J.L., Widmer F.
ISSN
0141-5492
Publication state
Published
Issued date
1979
Peer-reviewed
Oui
Volume
1
Number
3
Pages
109-114
Language
english
Abstract
Trypsin, pronase and subtilisin were immobilized on chitosan by glutaraldehyde coupling. Significant retention of activity was observed when synthetic substrates as well as casein were used. The specific activities of the bound proteinases ranged from 38% to 79% of their initial specific activities. The pH-activity profile of trypsin was slightly shifted toward alkaline values, and its thermal stability was increased. Immobilized trypsin was found to be less sensitive to its natural inhibitors than the soluble enzyme.
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13/08/2015 8:53
Last modification date
20/08/2019 15:12
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