Discovery of Plasmodium modulators by genome-wide analysis of circulating hemocytes in Anopheles gambiae.

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Version: Final published version
License: Not specified
Serval ID
serval:BIB_584DD0729C4B
Type
Article: article from journal or magazin.
Publication sub-type
Minutes: analyse of a published work.
Collection
Publications
Title
Discovery of Plasmodium modulators by genome-wide analysis of circulating hemocytes in Anopheles gambiae.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Pinto S.B., Lombardo F., Koutsos A.C., Waterhouse R.M., McKay K., An C., Ramakrishnan C., Kafatos F.C., Michel K.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
106
Number
50
Pages
21270-21275
Language
english
Abstract
Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. However, the molecular composition of these important immune cells has not been elucidated in depth, because of their scarcity in the circulating hemolymph, their adhesion to multiple tissues and the lack of primary culture methods to produce sufficient material for a genome-wide analysis. In this study, we report a genome-wide molecular characterization of circulating hemocytes collected from the hemolymph of adult female Anopheles gambiae mosquitoes--the major mosquito vector of human malaria in subSaharan Africa. Their molecular profile identified 1,485 transcripts with enriched expression in these cells, and many of these genes belong to innate immune gene families. This hemocyte-specific transcriptome is compared to those of Drosophila melanogaster and two other mosquitoes, Aedes aegypti and Armigeres subalbatus. We report the identification of two genes as ubiquitous hemocyte markers and several others as hemocyte subpopulation markers. We assess, via an RNAi screen, the roles in development of Plasmodium berghei of 63 genes expressed in hemocytes and provide a molecular comparison of the transcriptome of these cells during malaria infection.
Keywords
Aedes/genetics, Animals, Anopheles gambiae/genetics, Drosophila melanogaster/genetics, Female, Gene Expression Profiling, Gene Expression Regulation, Developmental, Genetic Markers, Genome, Insect/genetics, Hemocytes/metabolism, Malaria, Plasmodium, RNA, Messenger/analysis
Pubmed
Web of science
Open Access
Yes
Create date
20/09/2017 10:20
Last modification date
03/01/2020 18:21
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