Discovery of Plasmodium modulators by genome-wide analysis of circulating hemocytes in Anopheles gambiae.

Détails

Ressource 1Télécharger: PNAS-2009-Pinto-21270-5.pdf (965.34 [Ko])
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_584DD0729C4B
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Compte-rendu: analyse d'une oeuvre publiée.
Collection
Publications
Titre
Discovery of Plasmodium modulators by genome-wide analysis of circulating hemocytes in Anopheles gambiae.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Pinto S.B., Lombardo F., Koutsos A.C., Waterhouse R.M., McKay K., An C., Ramakrishnan C., Kafatos F.C., Michel K.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
106
Numéro
50
Pages
21270-21275
Langue
anglais
Résumé
Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. However, the molecular composition of these important immune cells has not been elucidated in depth, because of their scarcity in the circulating hemolymph, their adhesion to multiple tissues and the lack of primary culture methods to produce sufficient material for a genome-wide analysis. In this study, we report a genome-wide molecular characterization of circulating hemocytes collected from the hemolymph of adult female Anopheles gambiae mosquitoes--the major mosquito vector of human malaria in subSaharan Africa. Their molecular profile identified 1,485 transcripts with enriched expression in these cells, and many of these genes belong to innate immune gene families. This hemocyte-specific transcriptome is compared to those of Drosophila melanogaster and two other mosquitoes, Aedes aegypti and Armigeres subalbatus. We report the identification of two genes as ubiquitous hemocyte markers and several others as hemocyte subpopulation markers. We assess, via an RNAi screen, the roles in development of Plasmodium berghei of 63 genes expressed in hemocytes and provide a molecular comparison of the transcriptome of these cells during malaria infection.
Mots-clé
Aedes/genetics, Animals, Anopheles gambiae/genetics, Drosophila melanogaster/genetics, Female, Gene Expression Profiling, Gene Expression Regulation, Developmental, Genetic Markers, Genome, Insect/genetics, Hemocytes/metabolism, Malaria, Plasmodium, RNA, Messenger/analysis
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/09/2017 10:20
Dernière modification de la notice
03/01/2020 18:21
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