Effects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humans.

Details

Serval ID
serval:BIB_579D7E90276B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humans.
Journal
American Journal of Clinical Nutrition
Author(s)
Theytaz F., Noguchi Y., Egli L., Campos V., Buehler T., Hodson L., Patterson B.W., Nishikata N., Kreis R., Mittendorfer B., Fielding B., Boesch C., Tappy L.
ISSN
1938-3207 (Electronic)
ISSN-L
0002-9165
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
96
Number
5
Pages
1008-1016
Language
english
Notes
Publication types: Journal Article
Abstract
BACKGROUND: A high dietary protein intake has been shown to blunt the deposition of intrahepatic lipids in high-fat- and high-carbohydrate-fed rodents and humans.
OBJECTIVE: The aim of this study was to evaluate the effect of essential amino acid supplementation on the increase in hepatic fat content induced by a high-fructose diet in healthy subjects.
DESIGN: Nine healthy male volunteers were studied on 3 occasions in a randomized, crossover design after 6 d of dietary intervention. Dietary conditions consisted of a weight-maintenance balanced diet (control) or the same balanced diet supplemented with 3 g fructose · kg(-1) · d(-1) and 6.77 g of a mixture of 5 essential amino acids 3 times/d (leucine, isoleucine, valine, lysine, and threonine) (HFrAA) or with 3 g fructose · kg(-1) · d(-1) and a maltodextrin placebo 3 times/d (HFr); there was a washout period of 4 to 10 wk between each condition. For each condition, the intrahepatocellular lipid (IHCL) concentration, VLDL-triglyceride concentration, and VLDL-[(13)C]palmitate production were measured after oral loading with [(13)C]fructose.
RESULTS: HFr increased the IHCL content (1.27 ± 0.31 compared with 2.74 ± 0.55 vol %; P < 0.05) and VLDL-triglyceride (0.55 ± 0.06 compared with 1.40 ± 0.15 mmol/L; P < 0.05). HFr also enhanced VLDL-[(13)C]palmitate production. HFrAA significantly decreased IHCL compared with HFr (to 2.30 ± 0.43 vol%; P < 0.05) but did not change VLDL-triglyceride concentrations or VLDL-[(13)C]palmitate production.
CONCLUSIONS: Supplementation with essential amino acids blunts the fructose-induced increase in IHCL but not hypertriglyceridemia. This is not because of inhibition of VLDL-[(13)C]palmitate production. This trial was registered at www.clinicaltrials.gov as NCT01119989.
Pubmed
Web of science
Open Access
Yes
Create date
23/11/2012 21:23
Last modification date
20/08/2019 14:11
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