Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter.

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State: Public
Version: Final published version
Serval ID
serval:BIB_578DBAD79CB3
Type
Article: article from journal or magazin.
Collection
Publications
Title
Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter.
Journal
Nature communications
Author(s)
Klompmaker S.H., Kohl K., Fasel N., Mayer A.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
01/12/2017
Peer-reviewed
Oui
Volume
8
Number
1
Pages
1879
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Cells acquire free metals through plasma membrane transporters. But, in natural settings, sequestering agents often render metals inaccessible to transporters, limiting metal bioavailability. Here we identify a pathway for metal acquisition, allowing cells to cope with this situation. Under limited bioavailability of Mg <sup>2+</sup> , yeast cells upregulate fluid-phase endocytosis and transfer solutes from the environment into their vacuole, an acidocalcisome-like compartment loaded with highly concentrated polyphosphate. We propose that this anionic inorganic polymer, which is an avid chelator of Mg <sup>2+</sup> , serves as an immobilized cation filter that accumulates Mg <sup>2+</sup> inside these organelles. It thus allows the vacuolar exporter Mnr2 to efficiently transfer Mg <sup>2+</sup> into the cytosol. Leishmania parasites also employ acidocalcisomal polyphosphate to multiply in their Mg <sup>2+</sup> -limited habitat, the phagolysosomes of inflammatory macrophages. This suggests that the pathway for metal uptake via endocytosis, acidocalcisomal polyphosphates and export into the cytosol, which we term EAPEC, is conserved.
Keywords
Biological Transport, Cytosol/metabolism, Endocytosis, Leishmania/genetics, Leishmania/metabolism, Magnesium/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Vacuoles/genetics, Vacuoles/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
07/12/2017 21:28
Last modification date
20/08/2019 14:11
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