Robust synchronization of coupled circadian and cell cycle oscillators in single mammalian cells.

Details

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Serval ID
serval:BIB_54F704C349C4
Type
Article: article from journal or magazin.
Collection
Publications
Title
Robust synchronization of coupled circadian and cell cycle oscillators in single mammalian cells.
Journal
Molecular Systems Biology
Author(s)
Bieler J., Cannavo R., Gustafson K., Gobet C., Gatfield D., Naef F.
ISSN
1744-4292 (Electronic)
ISSN-L
1744-4292
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
10
Number
7
Pages
739
Language
english
Abstract
Circadian cycles and cell cycles are two fundamental periodic processes with a period in the range of 1 day. Consequently, coupling between such cycles can lead to synchronization. Here, we estimated the mutual interactions between the two oscillators by time-lapse imaging of single mammalian NIH3T3 fibroblasts during several days. The analysis of thousands of circadian cycles in dividing cells clearly indicated that both oscillators tick in a 1:1 mode-locked state, with cell divisions occurring tightly 5 h before the peak in circadian Rev-Erbα-YFP reporter expression. In principle, such synchrony may be caused by either unidirectional or bidirectional coupling. While gating of cell division by the circadian cycle has been most studied, our data combined with stochastic modeling unambiguously show that the reverse coupling is predominant in NIH3T3 cells. Moreover, temperature, genetic, and pharmacological perturbations showed that the two interacting cellular oscillators adopt a synchronized state that is highly robust over a wide range of parameters. These findings have implications for circadian function in proliferative tissues, including epidermis, immune cells, and cancer.
Keywords
cell cycle, circadian cycle, single cells, synchronization, time-lapse imaging
Pubmed
Web of science
Open Access
Yes
Create date
17/07/2014 11:21
Last modification date
08/05/2019 18:46
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