Biomechanical forces promote embryonic haematopoiesis.

Details

Serval ID
serval:BIB_540DECA3BE02
Type
Article: article from journal or magazin.
Collection
Publications
Title
Biomechanical forces promote embryonic haematopoiesis.
Journal
Nature
Author(s)
Adamo L., Naveiras O., Wenzel P.L., McKinney-Freeman S., Mack P.J., Gracia-Sancho J., Suchy-Dicey A., Yoshimoto M., Lensch M.W., Yoder M.C., García-Cardeña G., Daley G.Q.
ISSN
1476-4687 (Electronic)
ISSN-L
0028-0836
Publication state
Published
Issued date
25/06/2009
Peer-reviewed
Oui
Volume
459
Number
7250
Pages
1131-1135
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Biomechanical forces are emerging as critical regulators of embryogenesis, particularly in the developing cardiovascular system. After initiation of the heartbeat in vertebrates, cells lining the ventral aspect of the dorsal aorta, the placental vessels, and the umbilical and vitelline arteries initiate expression of the transcription factor Runx1 (refs 3-5), a master regulator of haematopoiesis, and give rise to haematopoietic cells. It remains unknown whether the biomechanical forces imposed on the vascular wall at this developmental stage act as a determinant of haematopoietic potential. Here, using mouse embryonic stem cells differentiated in vitro, we show that fluid shear stress increases the expression of Runx1 in CD41(+)c-Kit(+) haematopoietic progenitor cells, concomitantly augmenting their haematopoietic colony-forming potential. Moreover, we find that shear stress increases haematopoietic colony-forming potential and expression of haematopoietic markers in the para-aortic splanchnopleura/aorta-gonads-mesonephros of mouse embryos and that abrogation of nitric oxide, a mediator of shear-stress-induced signalling, compromises haematopoietic potential in vitro and in vivo. Collectively, these data reveal a critical role for biomechanical forces in haematopoietic development.
Keywords
Animals, Aorta/cytology, Aorta/embryology, Cell Differentiation, Cell Line, Cells, Cultured, Core Binding Factor Alpha 2 Subunit/genetics, Embryonic Stem Cells, Endothelium-Dependent Relaxing Factors/pharmacology, Female, Gene Expression Regulation, Developmental, Hematopoiesis/physiology, Hematopoietic Stem Cells/cytology, Hematopoietic Stem Cells/drug effects, Mice, Nitric Oxide/pharmacology, Pregnancy, Stress, Mechanical
Pubmed
Web of science
Create date
10/10/2022 23:04
Last modification date
11/10/2022 5:39
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