The effect of arachidonic acid on the hydroosmotic action of vasopressin in frog urinary bladder.

Details

Serval ID
serval:BIB_53FA7A036849
Type
Article: article from journal or magazin.
Collection
Publications
Title
The effect of arachidonic acid on the hydroosmotic action of vasopressin in frog urinary bladder.
Journal
Biochimica et Biophysica Acta. Molecular Cell Research
Author(s)
Firsov D.L., Parnova R.G.
ISSN
0167-4889
ISSN-L
0167-4889
Publication state
Published
Issued date
1994
Peer-reviewed
Oui
Volume
1220
Number
3
Pages
305-309
Language
english
Abstract
In experiments on frog urinary bladder it was found that 23 nM arginine-vasopressin (antidiuretic hormone, AVP), 10 min after addition, increased the content of free arachidonic acid (AA) in bladder tissue from 18.6 +/- 1.2 to 33.3 +/- 3.9 ng/mg protein. The exogenous AA (10(-8) - 10(-5) M) added to the serosal Ringer solution did not change the basal level of osmotic water flow, but significantly inhibited the hydroosmotic effect of 23 nM AVP in a dose-dependent manner. The inhibitory effect of AA was not eliminated in the presence of 1 microM indomethacin, 1 microM nordihydroguaiaretic acid, 100 microM ketoconazole, nor if the nonmetabolized analog of AA, eicosatetraynoic acid (1 microM), was used instead of AA. AA inhibited only the action of vasopressin, but had no effect on osmotic water flow induced by 2 mM cAMP + 50 microM 3-isobutyl-1-methylxanthine or 20 microM forskolin. These data suggest that AA is another phospholipid-derived messenger, which modulates the hydroosmotic action of vasopressin in frog urinary bladder at a point prior to cAMP formation.
Keywords
1-Methyl-3-isobutylxanthine/pharmacology, Animals, Arachidonic Acid/pharmacology, Arginine Vasopressin/pharmacology, Cyclic AMP/pharmacology, Drug Interactions, Fatty Acids, Nonesterified/metabolism, Forskolin/pharmacology, Kinetics, Rana temporaria, Urinary Bladder/drug effects, Urinary Bladder/metabolism
Pubmed
Web of science
Create date
24/01/2008 12:32
Last modification date
20/08/2019 14:09
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