Genomic and transcriptomic landscape of conjunctival melanoma.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_4F66F37907D8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Genomic and transcriptomic landscape of conjunctival melanoma.
Journal
PLoS genetics
Author(s)
Cisarova K., Folcher M., El Zaoui I., Pescini-Gobert R., Peter V.G., Royer-Bertrand B., Zografos L., Schalenbourg A., Nicolas M., Rimoldi D., Leyvraz S., Riggi N., Moulin A.P., Rivolta C.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Publication state
Published
Issued date
12/2020
Peer-reviewed
Oui
Volume
16
Number
12
Pages
e1009201
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Conjunctival melanoma (CJM) is a rare but potentially lethal and highly-recurrent cancer of the eye. Similar to cutaneous melanoma (CM), it originates from melanocytes. Unlike CM, however, CJM is relatively poorly characterized from a genomic point of view. To fill this knowledge gap and gain insight into the genomic nature of CJM, we performed whole-exome (WES) or whole-genome sequencing (WGS) of tumor-normal tissue pairs in 14 affected individuals, as well as RNA sequencing in a subset of 11 tumor tissues. Our results show that, similarly to CM, CJM is also characterized by a very high mutation load, composed of approximately 500 somatic mutations in exonic regions. This, as well as the presence of a UV light-induced mutational signature, are clear signs of the role of sunlight in CJM tumorigenesis. In addition, the genomic classification of CM proposed by TCGA seems to be well-applicable to CJM, with the presence of four typical subclasses defined on the basis of the most frequently mutated genes: BRAF, NF1, RAS, and triple wild-type. In line with these results, transcriptomic analyses revealed similarities with CM as well, namely the presence of a transcriptomic subtype enriched for immune genes and a subtype enriched for genes associated with keratins and epithelial functions. Finally, in seven tumors we detected somatic mutations in ACSS3, a possible new candidate oncogene. Transfected conjunctival melanoma cells overexpressing mutant ACSS3 showed higher proliferative activity, supporting the direct involvement of this gene in the tumorigenesis of CJM. Altogether, our results provide the first unbiased and complete genomic and transcriptomic classification of CJM.
Keywords
Cell Line, Tumor, Conjunctival Neoplasms/genetics, Conjunctival Neoplasms/metabolism, DNA Copy Number Variations, Female, Humans, Male, Melanoma/genetics, Melanoma/metabolism, Middle Aged, Mutation, Neurofibromin 1/genetics, Proto-Oncogene Proteins B-raf/genetics, Transcriptome, ras Proteins/genetics
Pubmed
Web of science
Open Access
Yes
Create date
04/01/2021 11:04
Last modification date
25/02/2021 7:09
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