Acute RyR1 Ca<sup>2+</sup> leak enhances NADH-linked mitochondrial respiratory capacity.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_4B4985CA147A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Acute RyR1 Ca<sup>2+</sup> leak enhances NADH-linked mitochondrial respiratory capacity.
Journal
Nature communications
Author(s)
Zanou N., Dridi H., Reiken S., Imamura de Lima T., Donnelly C., De Marchi U., Ferrini M., Vidal J., Sittenfeld L., Feige J.N., Garcia-Roves P.M., Lopez-Mejia I.C., Marks A.R., Auwerx J., Kayser B., Place N.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
10/12/2021
Peer-reviewed
Oui
Volume
12
Number
1
Pages
7219
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Sustained ryanodine receptor (RyR) Ca <sup>2+</sup> leak is associated with pathological conditions such as heart failure or skeletal muscle weakness. We report that a single session of sprint interval training (SIT), but not of moderate intensity continuous training (MICT), triggers RyR1 protein oxidation and nitrosylation leading to calstabin1 dissociation in healthy human muscle and in in vitro SIT models (simulated SIT or S-SIT). This is accompanied by decreased sarcoplasmic reticulum Ca <sup>2+</sup> content, increased levels of mitochondrial oxidative phosphorylation proteins, supercomplex formation and enhanced NADH-linked mitochondrial respiratory capacity. Mechanistically, (S-)SIT increases mitochondrial Ca <sup>2+</sup> uptake in mouse myotubes and muscle fibres, and decreases pyruvate dehydrogenase phosphorylation in human muscle and mouse myotubes. Countering Ca <sup>2+</sup> leak or preventing mitochondrial Ca <sup>2+</sup> uptake blunts S-SIT-induced adaptations, a result supported by proteomic analyses. Here we show that triggering acute transient Ca <sup>2+</sup> leak through RyR1 in healthy muscle may contribute to the multiple health promoting benefits of exercise.
Pubmed
Open Access
Yes
Create date
20/12/2021 14:24
Last modification date
24/12/2021 8:09
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