Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells.

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Serval ID
serval:BIB_49D577DC2FBF
Type
Article: article from journal or magazin.
Collection
Publications
Title
Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells.
Journal
Biotechnology Letters
Author(s)
Grosjean F., Bertschinger M., Hacker D.L., Wurm F.M.
ISSN
1573-6776 (Electronic)
ISSN-L
0141-5492
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
28
Number
22
Pages
1827-1833
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter genes. Also the exposure of a calcium phosphate-DNA coprecipitate to high osmolarity in vitro caused the release of the DNA from the precipitate. The results demonstrate the importance of the osmotic shock on the efficient delivery of plasmid DNA to the nucleus of CHO cells following calcium phosphate-mediated transfection.
Keywords
Animals, Biotechnology/methods, CHO Cells, Calcium Phosphates/chemistry, Cell Culture Techniques/methods, Cricetinae, Cricetulus, DNA/chemistry, Glycerol/chemistry, Microscopy, Ultraviolet, Osmosis, Osmotic Pressure, Plasmids/metabolism, Time Factors, Transfection
Pubmed
Web of science
Open Access
Yes
Create date
06/03/2015 11:47
Last modification date
01/10/2019 6:17
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