Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells.
Détails
Etat: Public
Version: Final published version
Licence: Non spécifiée
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
ID Serval
serval:BIB_49D577DC2FBF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells.
Périodique
Biotechnology Letters
ISSN
1573-6776 (Electronic)
ISSN-L
0141-5492
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
28
Numéro
22
Pages
1827-1833
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter genes. Also the exposure of a calcium phosphate-DNA coprecipitate to high osmolarity in vitro caused the release of the DNA from the precipitate. The results demonstrate the importance of the osmotic shock on the efficient delivery of plasmid DNA to the nucleus of CHO cells following calcium phosphate-mediated transfection.
Mots-clé
Animals, Biotechnology/methods, CHO Cells, Calcium Phosphates/chemistry, Cell Culture Techniques/methods, Cricetinae, Cricetulus, DNA/chemistry, Glycerol/chemistry, Microscopy, Ultraviolet, Osmosis, Osmotic Pressure, Plasmids/metabolism, Time Factors, Transfection
Pubmed
Web of science
Open Access
Oui
Création de la notice
06/03/2015 12:47
Dernière modification de la notice
14/02/2022 8:54
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