How to confirm C.E.R.A. doping in athletes' blood?

Details

Serval ID
serval:BIB_47D435872930
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
How to confirm C.E.R.A. doping in athletes' blood?
Journal
Forensic science international
Author(s)
Leuenberger N., Lamon S., Robinson N., Giraud S., Saugy M.
ISSN
1872-6283 (Electronic)
ISSN-L
0379-0738
Publication state
Published
Issued date
2011
Volume
213
Number
1-3
Pages
101-3
Language
english
Abstract
C.E.R.A. (Continuous Erythropoietin Receptor Activator) is a new third-generation erythropoiesis-stimulating agent that has recently been linked with abuse in endurance sports. The anti-doping community rapidly reacted by releasing a high-throughput screening ELISA allowing the detection of C.E.R.A. doping in athletes' blood. In order to return adverse analytical findings, anti-doping laboratories, however, need, as far as possible, to confirm the presence of the drug in athletes' samples through orthogonal methods. This article focuses on the comparison of 2 proposed confirmation assays based on gel electrophoresis that were coupled with a new sample immunopurification method. IEF, the classical method used to target erythropoietin (EPO) and its recombinant analogues in athletes' samples, and SARKOSYL-PAGE were applied to the plasma samples of subjects having received a single injection of C.E.R.A. It was demonstrated that SARKOSYL-PAGE was at least 6 times more sensitive than IEF, with comparable specificity. A longer detection window coupled with easier interpretation criteria led us to recommend the use of SARKOSYL-PAGE to confirm C.E.R.A. presence in athletes' blood.
Pubmed
Web of science
Create date
15/12/2011 16:19
Last modification date
20/08/2019 13:54
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