p53 Gene alterations and p53 protein accumulation in infiltrating ductal breast carcinomas: correlation between immunohistochemical and molecular biology techniques.

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_46B19A1F3DE7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
p53 Gene alterations and p53 protein accumulation in infiltrating ductal breast carcinomas: correlation between immunohistochemical and molecular biology techniques.
Journal
Modern pathology
Author(s)
Hurlimann J., Chaubert P., Benhattar J.
ISSN
0893-3952 (Print)
ISSN-L
0893-3952
Publication state
Published
Issued date
05/1994
Peer-reviewed
Oui
Volume
7
Number
4
Pages
423-428
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
One hundred and eighty-eight infiltrating ductal carcinomas of the breast were examined immunohistochemically (IMM) for p53, and the results were compared to those of single strand conformation polymorphism (SSCP). Of the 65 IMM+ cases (35%), 32 showed a genetic alteration in exons 5 to 9. In some of the IMM+ SSCP- cases, the number of 53+ cells in the tumor was too low to be detected by SSCP. Cases with only a few p53+ cells must not necessarily be considered negative, because a genetic alteration has been found in nine such cases. However, in a few cases, the accumulation of p53 protein could be caused by a factor other than mutation. Of the 123 IMM- cases, six showed gene polymorphism. p53 phenotype, as established with three monoclonal antibodies, did not correlate with genetic alteration in a particular exon. p53 IMM+ or SSCP+ tumors were generally ER-, grade III tumors and were uncommon in women older than 69 yr of age. The two methods have almost the same prognostic value. The accumulation of p53 protein is a good indicator of p53 mutation and therefore, immunohistochemistry remains a good method for the detection of such mutations.
Keywords
Adult, Age Factors, Aged, Aged, 80 and over, Base Sequence, Breast Neoplasms/chemistry, Breast Neoplasms/genetics, Breast Neoplasms/pathology, Carcinoma, Ductal, Breast/chemistry, Carcinoma, Ductal, Breast/genetics, Carcinoma, Ductal, Breast/pathology, DNA, Neoplasm/analysis, Exons, Female, Genes, p53/genetics, Humans, Immunohistochemistry, Lymphatic Metastasis, Middle Aged, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Genetic, Prognosis, Sensitivity and Specificity, Survival Analysis, Tumor Suppressor Protein p53/analysis
Pubmed
Web of science
Create date
29/01/2008 19:33
Last modification date
16/07/2020 9:42
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