Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro.

Details

Serval ID
serval:BIB_434F3B9DF831
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro.
Journal
FASEB Journal
Author(s)
Martina E., Degen M., Rüegg C., Merlo A., Lino M.M., Chiquet-Ehrismann R., Brellier F.
ISSN
1530-6860 (Electronic)
ISSN-L
0892-6638
Publication state
Published
Issued date
2010
Volume
24
Number
3
Pages
778-787
Language
english
Abstract
The microenvironment hosting a tumor actively participates in regulating tumor cell proliferation, migration, and invasion. Among the extracellular matrix proteins enriched in the stroma of carcinomas are the tenascin family members tenascin-C and tenascin-W. Whereas tenascin-C overexpression in gliomas is known to correlate with poor prognosis, the status of tenascin-W in brain tumors has not been investigated so far. In the present study, we analyzed protein levels of tenascin-W in 38 human gliomas and found expression of tenascin-W in 80% of the tumor samples, whereas no tenascin-W could be detected in control, nontumoral brain tissues. Double immunohistochemical staining of tenascin-W and von Willebrand factor revealed that tenascin-W is localized around blood vessels, exclusively in tumor samples. In vitro, the presence of tenascin-W increased the proportion of elongated human umbilical vein endothelial cells (HUVECs) and augmented the mean speed of cell migration. Furthermore, tenascin-W triggered sprouting of HUVEC spheroids to a similar extent as the proangiogenic factor tenascin-C. In conclusion, our study identifies tenascin-W as a candidate biomarker for brain tumor angiogenesis that could be used as a molecular target for therapy irrespective of the glioma subtype.-Martina, E., Degen, M., Rüegg, C., Merlo, A., Lino, M. M., Chiquet-Ehrismann, R., Brellier, F. Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro.
Keywords
Blood Vessels/metabolism, Blotting, Western, Cell Enlargement/drug effects, Cell Line, Cell Movement/drug effects, Cells, Cultured, Endothelial Cells/cytology, Endothelial Cells/drug effects, Fluorescent Antibody Technique, Glioma/metabolism, Humans, Neovascularization, Physiologic/drug effects, Spheroids, Cellular/drug effects, Tenascin/metabolism, Tenascin/pharmacology, Tumor Markers, Biological/metabolism, Tumor Markers, Biological/pharmacology, Umbilical Veins/cytology
Pubmed
Web of science
Create date
08/01/2010 15:57
Last modification date
20/08/2019 14:47
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