Reticulon 1-C/neuroendocrine-specific protein-C interacts with SNARE proteins
Details
Serval ID
serval:BIB_42C6481D31F9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Reticulon 1-C/neuroendocrine-specific protein-C interacts with SNARE proteins
Journal
Journal of Neurochemistry
ISSN
0022-3042 (Print)
Publication state
Published
Issued date
05/2004
Volume
89
Number
3
Pages
569-80
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Reticulons are proteins of neuroendocrine cells localized primarily to the endoplasmic reticulum membrane. Despite their implication in cellular processes like apoptosis or axonal regeneration, their intracellular molecular function is still largely unknown. Here, we show that reticulon 1-C can be detected in a protein complex of 150-200 kDa, and that a number of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins, i.e. syntaxin 1, syntaxin 7, syntaxin 13 and VAMP2, can be co-immunoprecipitated with reticulon 1-C. Moreover, it localizes to a nocodazole-sensitive, but calreticulin-negative domain of the endoplasmic reticulum. Finally, overexpression in PC12 cells of a reticulon 1-C fragment which binds to SNAREs, significantly enhances human growth hormone secretion. These results suggest that reticulons are involved in vesicle trafficking events, including regulated exocytosis.
Keywords
Animals
Antigens, Surface/metabolism
Cells, Cultured
Endoplasmic Reticulum/drug effects/metabolism
Exocytosis/physiology
Human Growth Hormone
Humans
Immune Sera/metabolism
Macromolecular Substances
Membrane Proteins/*metabolism
Nerve Tissue Proteins/*metabolism
Neurons/cytology/*metabolism
PC12 Cells
Precipitin Tests
Protein Binding/physiology
Protein Transport/physiology
Qa-SNARE Proteins
R-SNARE Proteins
Rats
SNARE Proteins
Syntaxin 1
*Vesicular Transport Proteins
Pubmed
Web of science
Create date
24/01/2008 15:30
Last modification date
20/08/2019 14:45