Reticulon 1-C/neuroendocrine-specific protein-C interacts with SNARE proteins

Details

Serval ID
serval:BIB_42C6481D31F9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Reticulon 1-C/neuroendocrine-specific protein-C interacts with SNARE proteins
Journal
Journal of Neurochemistry
Author(s)
Steiner  P., Kulangara  K., Sarria  J. C., Glauser  L., Regazzi  R., Hirling  H.
ISSN
0022-3042 (Print)
Publication state
Published
Issued date
05/2004
Volume
89
Number
3
Pages
569-80
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Reticulons are proteins of neuroendocrine cells localized primarily to the endoplasmic reticulum membrane. Despite their implication in cellular processes like apoptosis or axonal regeneration, their intracellular molecular function is still largely unknown. Here, we show that reticulon 1-C can be detected in a protein complex of 150-200 kDa, and that a number of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins, i.e. syntaxin 1, syntaxin 7, syntaxin 13 and VAMP2, can be co-immunoprecipitated with reticulon 1-C. Moreover, it localizes to a nocodazole-sensitive, but calreticulin-negative domain of the endoplasmic reticulum. Finally, overexpression in PC12 cells of a reticulon 1-C fragment which binds to SNAREs, significantly enhances human growth hormone secretion. These results suggest that reticulons are involved in vesicle trafficking events, including regulated exocytosis.
Keywords
Animals Antigens, Surface/metabolism Cells, Cultured Endoplasmic Reticulum/drug effects/metabolism Exocytosis/physiology Human Growth Hormone Humans Immune Sera/metabolism Macromolecular Substances Membrane Proteins/*metabolism Nerve Tissue Proteins/*metabolism Neurons/cytology/*metabolism PC12 Cells Precipitin Tests Protein Binding/physiology Protein Transport/physiology Qa-SNARE Proteins R-SNARE Proteins Rats SNARE Proteins Syntaxin 1 *Vesicular Transport Proteins
Pubmed
Web of science
Create date
24/01/2008 14:30
Last modification date
20/08/2019 13:45
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