Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model.

Details

Serval ID
serval:BIB_416DCA6C7AC6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model.
Journal
Cellular Microbiology
Author(s)
Alibaud L., Köhler T., Coudray A., Prigent-Combaret C., Bergeret E., Perrin J., Benghezal M., Reimmann C., Gauthier Y., van Delden C., Attree I., Fauvarque M.O., Cosson P.
ISSN
1462-5822[electronic], 1462-5814[linking]
Publication state
Published
Issued date
2008
Volume
10
Number
3
Pages
729-740
Language
english
Abstract
The human pathogen Pseudomonas aeruginosa has been shown previously to use similar virulence factors when infecting mammalian hosts or Dictyostelium amoebae. Here we randomly mutagenized a clinical isolate of P. aeruginosa, and identified mutants with attenuated virulence towards Dictyostelium. These mutant strains also exhibited a strong decrease in virulence when infecting Drosophila and mice, confirming that P. aeruginosa makes use of similar virulence traits to confront these very different hosts. Further characterization of these bacterial mutants showed that TrpD is important for the induction of the quorum-sensing circuit, while PchH and PchI are involved in the induction of the type III secretion system. These results demonstrate the usefulness and the relevance of the Dictyostelium host model to identify and analyse new virulence genes in P. aeruginosa.
Keywords
ATP-Binding Cassette Transporters/genetics, ATP-Binding Cassette Transporters/physiology, Animals, Bacterial Proteins/genetics, Bacterial Proteins/physiology, DNA Transposable Elements, Dictyostelium/microbiology, Drosophila, Female, Humans, Male, Mice, Mutagenesis, Insertional, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/pathogenicity, Survival Analysis, Virulence/genetics, Virulence Factors/genetics
Pubmed
Web of science
Open Access
Yes
Create date
03/03/2008 15:39
Last modification date
20/08/2019 14:41
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