Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Details

Serval ID
serval:BIB_40678CC312D4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection
Journal
Optics letters
Author(s)
Kühn J., Montfort F., Colomb T., Rappaz B., Moratal C., Pavillon N., Marquet P., Depeursinge C.
ISSN
0146-9592
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
34
Number
5
Pages
653-655
Language
english
Notes
Publication types: Journal Article - Publication Status: ppublish
Abstract
We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 mum axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.
Pubmed
Web of science
Create date
13/03/2009 10:57
Last modification date
20/08/2019 13:38
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