An overview of the metabolic differences between Bradyrhizobium japonicum 110 bacteria and differentiated bacteroids from soybean (Glycine max) root nodules: An in vitro 13C-and 31P-nuclear magnetic resonance spectroscopy study

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State: Public
Version: author
Serval ID
serval:BIB_3F1A9A9F73B1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An overview of the metabolic differences between Bradyrhizobium japonicum 110 bacteria and differentiated bacteroids from soybean (Glycine max) root nodules: An in vitro 13C-and 31P-nuclear magnetic resonance spectroscopy study
Journal
FEMS Microbiology Letters
Author(s)
Vauclare P., Bligny R., Gout E., Widmer F.
ISSN
0378-1097
ISSN-L
0378-1097
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
343
Number
1
Pages
49-56
Language
english
Abstract
Bradyrhizobium japonicum is a symbiotic nitrogen-fixing soil bacteria that induce root nodules formation in legume soybean (Glycine max.). Using 13C- and 31P-nuclear magnetic resonance (NMR) spectroscopy, we have analysed the metabolite profiles of cultivated B.japonicum cells and bacteroids isolated from soybean nodules. Our results revealed some quantitative and qualitative differences between the metabolite profiles of bacteroids and their vegetative state. This includes in bacteroids a huge accumulation of soluble carbohydrates such as trehalose, glutamate, myo-inositol and homospermidine as well as Pi, nucleotide pools and intermediates of the primary carbon metabolism. Using this novel approach, these data show that most of the compounds detected in bacteroids reflect the metabolic adaptation of rhizobia to the surrounding microenvironment with its host plant cells.
Keywords
metabolism, NMR, Bradyrhizobium japonicum, rhizobium-legume symbiosis, nitrogen-fixation, plant-cells, accumulation, glutamate, meliloti, betaine, oxygen, dehydrogenase, polyamines, Microbiology
Pubmed
Web of science
Open Access
Yes
Create date
16/09/2015 10:13
Last modification date
20/08/2019 13:36
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