Localized in vivo 13C-NMR of glutamate metabolism in the human brain: initial results at 4 tesla.

Details

Serval ID
serval:BIB_3EAEF88E8B9E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Localized in vivo 13C-NMR of glutamate metabolism in the human brain: initial results at 4 tesla.
Journal
Developmental Neuroscience
Author(s)
Gruetter R., Seaquist E.R., Kim S., Ugurbil K.
ISSN
0378-5866 (Print)
ISSN-L
0378-5866
Publication state
Published
Issued date
1998
Volume
20
Number
4-5
Pages
380-388
Language
english
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
Using optimized administration of 13C-labeled glucose, the time course of the specific activity of glucose was measured directly by in vivo 13C-NMR in the human brain at 4 Tesla. Subsequent label incorporation was measured at the C2, C3 and C4 positions of both glutamate and the well-resolved C2, C3 and C4 resonances of glutamine and at the C2 and C3 positions of aspartate. GABA was clearly observed for the first time in vivo, suggesting a substantial GABA turnover in the normal human visual cortex. Likewise, lactate C3 labeled with an estimated active pool size on the order of 0.5 mM. A model of cerebral glutamate metabolism is proposed which predicts that glutamatergic action ('neurotransmission'), pyruvate carboxylase flux, TCA cycle activity, glucose consumption and exchange across the mitochondrial membrane can be assessed simultaneously in the human brain.
Keywords
Aspartic Acid/analogs & derivatives, Aspartic Acid/metabolism, Blood Glucose/metabolism, Brain/metabolism, Carbon Isotopes, Glucose/metabolism, Glutamic Acid/metabolism, Glutamine/metabolism, Humans, Lactic Acid/metabolism, Magnetic Resonance Spectroscopy, Magnetics, Models, Biological, Visual Cortex/metabolism, gamma-Aminobutyric Acid/metabolism
Pubmed
Web of science
Create date
04/08/2010 15:28
Last modification date
20/08/2019 13:35
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