Differential effect of interleukin-4 and transforming growth factor beta 1 on expression of proto-oncogenes and autocrine insulin-like growth factor II in colorectal carcinoma cells

Details

Serval ID
serval:BIB_3AF957565647
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential effect of interleukin-4 and transforming growth factor beta 1 on expression of proto-oncogenes and autocrine insulin-like growth factor II in colorectal carcinoma cells
Journal
Biochemical and Biophysical Research Communications
Author(s)
Lahm  H., Amstad  P., Yilmaz  A., Fischer  J. R., Givel  J. C., Odartchenko  N., Sordat  B.
ISSN
0006-291X (Print)
Publication state
Published
Issued date
03/1996
Volume
220
Number
2
Pages
334-40
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 18
Abstract
We have compared the effect of interleukin-4 and transforming growth factor beta 1 on proliferation and gene expression in two colorectal carcinoma cell lines, LS513 and LS1034. Transforming growth factor beta 1 was a potent inhibitor for both cells lines and virtually abolished de novo DNA synthesis. Interleukin-4 inhibited thymidine incorporation up to 60 and 45%, respectively. While both cytokines exerted a comparable cyto-inhibitory activity they displayed differential effects on proto-oncogene expression. Transforming growth factor beta 1 markedly down-regulated c-myc in LS1034 but not in LS513 cells. In contrast, expression of c-fos was induced by interleukin-4 in LS513 but not in LS1034 cells. Interestingly, in agreement with their cyto-inhibitory activity both cytokines suppressed the expression of insulin-like growth factor II in LS1034, which is an autocrine growth factor for these cells.
Keywords
Cell Division Colorectal Neoplasms/*genetics/metabolism/pathology *Gene Expression Genes, fos/genetics Genes, myc/genetics Humans Insulin-Like Growth Factor II/*genetics Interleukin-4/*pharmacology Proto-Oncogenes/*genetics Transforming Growth Factor beta/*pharmacology Tumor Cells, Cultured
Pubmed
Web of science
Create date
28/01/2008 8:56
Last modification date
20/08/2019 13:30
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