Differential effect of interleukin-4 and transforming growth factor beta 1 on expression of proto-oncogenes and autocrine insulin-like growth factor II in colorectal carcinoma cells
Details
Serval ID
serval:BIB_3AF957565647
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential effect of interleukin-4 and transforming growth factor beta 1 on expression of proto-oncogenes and autocrine insulin-like growth factor II in colorectal carcinoma cells
Journal
Biochemical and Biophysical Research Communications
ISSN
0006-291X (Print)
Publication state
Published
Issued date
03/1996
Volume
220
Number
2
Pages
334-40
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 18
Research Support, Non-U.S. Gov't --- Old month value: Mar 18
Abstract
We have compared the effect of interleukin-4 and transforming growth factor beta 1 on proliferation and gene expression in two colorectal carcinoma cell lines, LS513 and LS1034. Transforming growth factor beta 1 was a potent inhibitor for both cells lines and virtually abolished de novo DNA synthesis. Interleukin-4 inhibited thymidine incorporation up to 60 and 45%, respectively. While both cytokines exerted a comparable cyto-inhibitory activity they displayed differential effects on proto-oncogene expression. Transforming growth factor beta 1 markedly down-regulated c-myc in LS1034 but not in LS513 cells. In contrast, expression of c-fos was induced by interleukin-4 in LS513 but not in LS1034 cells. Interestingly, in agreement with their cyto-inhibitory activity both cytokines suppressed the expression of insulin-like growth factor II in LS1034, which is an autocrine growth factor for these cells.
Keywords
Cell Division
Colorectal Neoplasms/*genetics/metabolism/pathology
*Gene Expression
Genes, fos/genetics
Genes, myc/genetics
Humans
Insulin-Like Growth Factor II/*genetics
Interleukin-4/*pharmacology
Proto-Oncogenes/*genetics
Transforming Growth Factor beta/*pharmacology
Tumor Cells, Cultured
Pubmed
Web of science
Create date
28/01/2008 9:56
Last modification date
20/08/2019 14:30