Yeast-to-hypha transition of Schizosaccharomyces japonicus in response to environmental stimuli.

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State: Public
Version: Final published version
License: CC BY-NC 4.0
Serval ID
serval:BIB_3A8D90BECDB6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Yeast-to-hypha transition of Schizosaccharomyces japonicus in response to environmental stimuli.
Journal
Molecular biology of the cell
Author(s)
Kinnaer C., Dudin O., Martin S.G.
ISSN
1939-4586 (Electronic)
ISSN-L
1059-1524
Publication state
Published
Issued date
01/04/2019
Peer-reviewed
Oui
Volume
30
Number
8
Pages
975-991
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Many fungal species are dimorphic, exhibiting both unicellular yeast-like and filamentous forms. Schizosaccharomyces japonicus, a member of the fission yeast clade, is one such dimorphic fungus. Here, we first identify fruit extracts as natural, stress-free, starvation-independent inducers of filamentation, which we use to describe the properties of the dimorphic switch. During the yeast-to-hypha transition, the cell evolves from a bipolar to a unipolar system with 10-fold accelerated polarized growth but constant width, vacuoles segregated to the nongrowing half of the cell, and hyper-lengthening of the cell. We demonstrate unusual features of S. japonicus hyphae: these cells lack a Spitzenkörper, a vesicle distribution center at the hyphal tip, but display more rapid cytoskeleton-based transport than the yeast form, with actin cables being essential for the transition. S. japonicus hyphae also remain mononuclear and undergo complete cell divisions, which are highly asymmetric: one daughter cell inherits the vacuole, the other the growing tip. We show that these elongated cells scale their nuclear size, spindle length, and elongation rates, but display altered division size controls. This establishes S. japonicus as a unique system that switches between symmetric and asymmetric modes of growth and division.
Keywords
Cell Cycle/genetics, Cell Division/genetics, Cytoskeleton/physiology, Fungal Proteins/genetics, Fungi/cytology, Hyphae/cytology, Microtubules/physiology, Schizosaccharomyces/cytology, Schizosaccharomyces/metabolism, Vacuoles/physiology
Pubmed
Web of science
Open Access
Yes
Create date
18/03/2019 18:15
Last modification date
18/01/2020 7:08
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