Heterogeneity assessment of functional T cell avidity.

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Version: Final published version
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Serval ID
serval:BIB_39C885ED43A7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Heterogeneity assessment of functional T cell avidity.
Journal
Scientific reports
Author(s)
Ioannidou K., Baumgaertner P., Gannon P.O., Speiser M.F., Allard M., Hebeisen M., Rufer N., Speiser D.E.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
13/03/2017
Peer-reviewed
Oui
Volume
7
Pages
44320
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The potency of cellular immune responses strongly depends on T cell avidity to antigen. Yet, functional avidity measurements are rarely performed in patients, mainly due to the technical challenges of characterizing heterogeneous T cells. The mean functional T cell avidity can be determined by the IFN-γ Elispot assay, with titrated amounts of peptide. Using this assay, we developed a method revealing the heterogeneity of functional avidity, represented by the steepness/hillslope of the peptide titration curve, documented by proof of principle experiments and mathematical modeling. Our data show that not only natural polyclonal CD8 T cell populations from cancer patients, but also monoclonal T cells differ strongly in their heterogeneity of functional avidity. Interestingly, clones and polyclonal cells displayed comparable ranges of heterogeneity. We conclude that besides the mean functional avidity, it is feasible and useful to determine its heterogeneity (hillslope) for characterizing T cell responses in basic research and patient investigation.
Keywords
CD8-Positive T-Lymphocytes/cytology, CD8-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/metabolism, Cell Survival/immunology, Clone Cells/immunology, Clone Cells/metabolism, Enzyme-Linked Immunospot Assay/methods, Epitopes, T-Lymphocyte/immunology, Feasibility Studies, Humans, Immunity, Cellular/immunology, Interferon-gamma/immunology, Interferon-gamma/metabolism, Lymphocyte Activation/immunology, Reproducibility of Results
Pubmed
Web of science
Open Access
Yes
Create date
21/03/2017 19:18
Last modification date
02/04/2020 6:19
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