Hepatitis C virus nonstructural protein 4B: a journey into unexplored territory.

Details

Serval ID
serval:BIB_39AB8AFB299B
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Hepatitis C virus nonstructural protein 4B: a journey into unexplored territory.
Journal
Reviews in Medical Virology
Author(s)
Gouttenoire Jerome, Penin Francois, Moradpour Darius
ISSN
1099-1654[electronic], 1052-9276[linking]
Publication state
Published
Issued date
2010
Volume
20
Number
2
Pages
117-129
Language
english
Abstract
Hepatitis C virus (HCV) is a positive-strand RNA virus that replicates its genome in a membrane-associated replication complex. Nonstructural protein 4B (NS4B) induces the specific membrane alteration, designated as membranous web (MW), that harbours this complex. HCV NS4B is an integral membrane protein predicted to comprise four transmembrane segments in its central part. The N-terminal part comprises two amphipathic alpha-helices of which the second has the potential to traverse the membrane bilayer, likely upon oligomerisation. The C-terminal part comprises a predicted highly conserved alpha-helix, a membrane-associated amphipathic alpha-helix and two reported palmitoylation sites. NS4B interacts with other viral nonstructural proteins and has been reported to bind viral RNA. In addition, it was found to harbour an NTPase activity. Finally, NS4B has recently been found to have a role in viral assembly. Much work needs to be done with respect to further dissecting these multiple functions as well as providing a refined membrane topology and complete structure of NS4B. Progress in this direction should yield important insights into the functional architecture of the HCV replication complex and may reveal new opportunities for antiviral intervention against a leading cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma worldwide.
Keywords
Dependent Rna-Polymerase, Endoplasmic-Reticulum Membrane, Nucleotide-Binding Motif, NS4B Protein, Cell-Culture, Infectious Virus, Replication Complex, Core Protein, Dengue Virus, Subgenomic Replicons
Pubmed
Web of science
Create date
13/04/2010 10:47
Last modification date
20/08/2019 14:29
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