Hepatitis C virus nonstructural protein 4B: a journey into unexplored territory.

Détails

ID Serval
serval:BIB_39AB8AFB299B
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Hepatitis C virus nonstructural protein 4B: a journey into unexplored territory.
Périodique
Reviews in Medical Virology
Auteur(s)
Gouttenoire Jerome, Penin Francois, Moradpour Darius
ISSN
1099-1654[electronic], 1052-9276[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
20
Numéro
2
Pages
117-129
Langue
anglais
Résumé
Hepatitis C virus (HCV) is a positive-strand RNA virus that replicates its genome in a membrane-associated replication complex. Nonstructural protein 4B (NS4B) induces the specific membrane alteration, designated as membranous web (MW), that harbours this complex. HCV NS4B is an integral membrane protein predicted to comprise four transmembrane segments in its central part. The N-terminal part comprises two amphipathic alpha-helices of which the second has the potential to traverse the membrane bilayer, likely upon oligomerisation. The C-terminal part comprises a predicted highly conserved alpha-helix, a membrane-associated amphipathic alpha-helix and two reported palmitoylation sites. NS4B interacts with other viral nonstructural proteins and has been reported to bind viral RNA. In addition, it was found to harbour an NTPase activity. Finally, NS4B has recently been found to have a role in viral assembly. Much work needs to be done with respect to further dissecting these multiple functions as well as providing a refined membrane topology and complete structure of NS4B. Progress in this direction should yield important insights into the functional architecture of the HCV replication complex and may reveal new opportunities for antiviral intervention against a leading cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma worldwide.
Mots-clé
Dependent Rna-Polymerase, Endoplasmic-Reticulum Membrane, Nucleotide-Binding Motif, NS4B Protein, Cell-Culture, Infectious Virus, Replication Complex, Core Protein, Dengue Virus, Subgenomic Replicons
Pubmed
Web of science
Création de la notice
13/04/2010 10:47
Dernière modification de la notice
20/08/2019 14:29
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