(MA)LDI MS Imaging at High Specificity and Sensitivity

Details

Serval ID
serval:BIB_391B5365B47F
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
(MA)LDI MS Imaging at High Specificity and Sensitivity
Title of the book
Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry
Author(s)
Thomas A., Heath Patterson N., Dufresne M., Chaurand P.
Publisher
Springer
ISBN
9783319048185
Publication state
Published
Issued date
2016
Pages
129-147
Language
english
Abstract
(Matrix-assisted) laser desorption/ionization ((MA)LDI) mass spectrometry imaging (MSI) has been driven by remarkable technological developments in the last couple of years. Although molecular information of a wide range of molecules including peptides, lipids, metabolites, and xenobiotics can be mapped, (MA)LDI MSI only leads to the detection of the most abundant soluble molecules in the cells and, consequently, does not provide access to the least expressed species, which can be very informative in the scope of disease research. Within a short period of time, numerous protocols and concepts have been developed and introduced in order to increase MSI sensitivity, including in situ tissue chemistry and solvent-free matrix depositions. In this chapter, we will discuss some of the latest developments in the field of high-sensitivity MSI using solvent-free matrix depositions and will detail protocols of two methods with their capability of enriching molecular MSI signal as demonstrated within our laboratory.
Keywords
MALDI Mass spectrometry Imaging Tissue 1,5-diaminonaphtalene Sublimation Silver Sputtering Specificity Sensitivity Lipids Fatty acids Cholesterol
Create date
05/01/2016 8:57
Last modification date
20/08/2019 13:28
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