SosA inhibits cell division in Staphylococcus aureus in response to DNA damage.

Details

Serval ID
serval:BIB_37C40301ACDA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
SosA inhibits cell division in Staphylococcus aureus in response to DNA damage.
Journal
Molecular microbiology
Author(s)
Bojer M.S., Wacnik K., Kjelgaard P., Gallay C., Bottomley A.L., Cohn M.T., Lindahl G., Frees D., Veening J.W., Foster S.J., Ingmer H.
ISSN
1365-2958 (Electronic)
ISSN-L
0950-382X
Publication state
Published
Issued date
10/2019
Peer-reviewed
Oui
Volume
112
Number
4
Pages
1116-1130
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Inhibition of cell division is critical for viability under DNA-damaging conditions. DNA damage induces the SOS response that in bacteria inhibits cell division while repairs are being made. In coccoids, such as the human pathogen, Staphylococcus aureus, this process remains poorly studied. Here, we identify SosA as the staphylococcal SOS-induced cell division inhibitor. Overproduction of SosA inhibits cell division, while sosA inactivation sensitizes cells to genotoxic stress. SosA is a small, predicted membrane protein with an extracellular C-terminal domain in which point mutation of residues that are conserved in staphylococci and major truncations abolished the inhibitory activity. In contrast, a minor truncation led to SosA accumulation and a strong cell division inhibitory activity, phenotypically similar to expression of wild-type SosA in a CtpA membrane protease mutant. This suggests that the extracellular C-terminus of SosA is required both for cell division inhibition and for turnover of the protein. Microscopy analysis revealed that SosA halts cell division and synchronizes the cell population at a point where division proteins such as FtsZ and EzrA are localized at midcell, and the septum formation is initiated but unable to progress to closure. Thus, our findings show that SosA is central in cell division regulation in staphylococci.
Keywords
Bacterial Proteins/metabolism, Cell Division/genetics, Cell Division/physiology, Cytoskeletal Proteins/metabolism, DNA Damage/genetics, DNA Damage/physiology, Membrane Proteins/metabolism, SOS Response, Genetics/genetics, SOS Response, Genetics/physiology, Staphylococcal Infections/metabolism, Staphylococcus aureus/genetics, Staphylococcus aureus/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
21/07/2019 15:47
Last modification date
20/06/2020 6:18
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