Analysis of interaction of cloned human and/or sheep fetal hemoglobin gamma-chain and LPS in augmenting induction of inflammatory cytokine production in vivo and in vitro.
Details
Serval ID
serval:BIB_37776
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of interaction of cloned human and/or sheep fetal hemoglobin gamma-chain and LPS in augmenting induction of inflammatory cytokine production in vivo and in vitro.
Journal
Immunology Letters
ISSN
0165-2478 (Print)
ISSN-L
0165-2478
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
100
Number
2
Pages
120-129
Language
english
Abstract
We have reported earlier that purified preparations of sheep fetal hemoglobin, but not adult hemoglobin, in concert with non-stimulatory doses of lipopolysaccharide (LPS) (lipid A), act cooperatively to regulate in vitro production of a number of cytokines, including TNFalpha, TGFbeta and IL-6 from murine and human leukocytes. Following in vivo treatment of mice with the same combination of hemoglobin and LPS, harvested spleen or peritoneal cells showed a similar augmented capacity to release these cytokines into culture supernatants. We report below that genetically cloned gamma-chain of human or sheep fetal hemoglobin, but not cloned alpha- or beta-chains, can produce this cooperative effect, as indeed can HPLC purified, heme-free, gamma-chains derived from cord blood fetal hemoglobin, and that purified haptoglobin completely abolishes the cooperative interaction.
Keywords
Age Factors, Amino Acid Sequence, Animals, Cloning, Molecular, Cricetinae, Dose-Response Relationship, Drug, Fetal Hemoglobin/biosynthesis, Fetal Hemoglobin/genetics, Globins/biosynthesis, Globins/chemistry, Haptoglobins/pharmacology, Humans, Interleukin-6/biosynthesis, Lipid A/administration & dosage, Lipid A/antagonists & inhibitors, Lipopolysaccharides/administration & dosage, Lipopolysaccharides/immunology, Lymphocytes/drug effects, Mice, Molecular Sequence Data, Sheep, Spleen/cytology, Spleen/drug effects, Transforming Growth Factor beta/biosynthesis, Tumor Necrosis Factor-alpha/biosynthesis
OAI-PMH
Pubmed
Web of science
Create date
19/11/2007 13:36
Last modification date
20/08/2019 14:25