Analysis of interaction of cloned human and/or sheep fetal hemoglobin gamma-chain and LPS in augmenting induction of inflammatory cytokine production in vivo and in vitro.

Details

Serval ID
serval:BIB_37776
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of interaction of cloned human and/or sheep fetal hemoglobin gamma-chain and LPS in augmenting induction of inflammatory cytokine production in vivo and in vitro.
Journal
Immunology Letters
Author(s)
Gorczynski R.M., Alexander C., Bessler W., Fournier K., Hoffmann P., Mach J.P., Rietschel E.T., Song L., Waelli T., Westphal O., Zahringer U., Khatri I.
ISSN
0165-2478 (Print)
ISSN-L
0165-2478
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
100
Number
2
Pages
120-129
Language
english
Abstract
We have reported earlier that purified preparations of sheep fetal hemoglobin, but not adult hemoglobin, in concert with non-stimulatory doses of lipopolysaccharide (LPS) (lipid A), act cooperatively to regulate in vitro production of a number of cytokines, including TNFalpha, TGFbeta and IL-6 from murine and human leukocytes. Following in vivo treatment of mice with the same combination of hemoglobin and LPS, harvested spleen or peritoneal cells showed a similar augmented capacity to release these cytokines into culture supernatants. We report below that genetically cloned gamma-chain of human or sheep fetal hemoglobin, but not cloned alpha- or beta-chains, can produce this cooperative effect, as indeed can HPLC purified, heme-free, gamma-chains derived from cord blood fetal hemoglobin, and that purified haptoglobin completely abolishes the cooperative interaction.
Keywords
Age Factors, Amino Acid Sequence, Animals, Cloning, Molecular, Cricetinae, Dose-Response Relationship, Drug, Fetal Hemoglobin/biosynthesis, Fetal Hemoglobin/genetics, Globins/biosynthesis, Globins/chemistry, Haptoglobins/pharmacology, Humans, Interleukin-6/biosynthesis, Lipid A/administration & dosage, Lipid A/antagonists & inhibitors, Lipopolysaccharides/administration & dosage, Lipopolysaccharides/immunology, Lymphocytes/drug effects, Mice, Molecular Sequence Data, Sheep, Spleen/cytology, Spleen/drug effects, Transforming Growth Factor beta/biosynthesis, Tumor Necrosis Factor-alpha/biosynthesis
Pubmed
Web of science
Create date
19/11/2007 13:36
Last modification date
20/08/2019 14:25
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