Analysis of teichoic acid biosynthesis regulation reveals that the extracytoplasmic function sigma factor sigmaM is induced by phosphate depletion in Bacillus subtilis W23.

Details

Serval ID
serval:BIB_37326
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of teichoic acid biosynthesis regulation reveals that the extracytoplasmic function sigma factor sigmaM is induced by phosphate depletion in Bacillus subtilis W23.
Journal
Microbiology
Author(s)
Minnig K., Lazarevic V., Soldo B., Mauël C.
ISSN
1350-0872[print], 1350-0872[linking]
Publication state
Published
Issued date
2005
Volume
151
Number
Pt 9
Pages
3041-3049
Language
english
Abstract
The expression of the Bacillus subtilis W23 tar genes specifying the biosynthesis of the major wall teichoic acid, the poly(ribitol phosphate), was studied under phosphate limitation using lacZ reporter fusions. Three different regulation patterns can be deduced from these beta-galactosidase activity data: (i) tarD and tarL gene expression is downregulated under phosphate starvation; (ii) tarA and, to a minor extent, tarB expression after an initial decrease unexpectedly increases; and (iii) tarO is not influenced by phosphate concentration. To dissect the tarA regulatory pattern, its two promoters were analysed under phosphate limitation: The P(tarA)-ext promoter is repressed under phosphate starvation by the PhoPR two-component system, whereas, under the same conditions, the P(tarA)-int promoter is upregulated by the action of an extracytoplasmic function (ECF) sigma factor, sigma(M). In contrast to strain 168, sigma(M) is activated in strain W23 in phosphate-depleted conditions, a phenomenon indirectly dependent on PhoPR, the two-component regulatory system responsible for the adaptation to phosphate starvation. These results provide further evidence for the role of sigma(M) in cell-wall stress response, and suggest that impairment of cell-wall structure is the signal activating this ECF sigma factor.
Keywords
Bacillus subtilis/genetics, Bacillus subtilis/metabolism, Bacterial Proteins, Cell Wall/metabolism, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Phosphates/deficiency, Phosphates/metabolism, Sigma Factor/metabolism, Teichoic Acids/biosynthesis, Teichoic Acids/genetics, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:35
Last modification date
20/08/2019 13:25
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