Establishing two-dimensional gels for the analysis of Leishmania proteomes.

Details

Serval ID
serval:BIB_33D358FBBC78
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Establishing two-dimensional gels for the analysis of Leishmania proteomes.
Journal
Proteomics
Author(s)
Acestor N., Masina S., Walker J., Saravia N.G., Fasel N., Quadroni M.
ISSN
1615-9853 (Print)
ISSN-L
1615-9853
Publication state
Published
Issued date
2002
Volume
2
Number
7
Pages
877-879
Language
english
Abstract
Several different sample preparation methods for two-dimensional electrophoresis (2-DE) analysis of Leishmania parasites were compared. From this work, we were able to identify a solubilization method using Nonidet P-40 as detergent, which was simple to follow, and which produced 2-DE gels of high resolution and reproducibility.
Keywords
Animals, Electrophoresis, Gel, Two-Dimensional/methods, Leishmania/chemistry, Leishmania/genetics, Molecular Weight, Proteome/chemistry, Protozoan Proteins/analysis
Pubmed
Web of science
Create date
24/01/2008 16:02
Last modification date
20/08/2019 14:20
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