In vitro and in vivo effectiveness of an innovative silver-copper nanoparticle coating of catheters to prevent methicillin-resistant Staphylococcus aureus infection

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State: Public
Version: author
Serval ID
serval:BIB_331CBDC800C0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vitro and in vivo effectiveness of an innovative silver-copper nanoparticle coating of catheters to prevent methicillin-resistant Staphylococcus aureus infection
Journal
Antimicrobial Agents and Chemotherapy
Author(s)
Ballo Myriam K.S., Rtimi Sami, Pulgarin César, Hopf Nancy, Berthet Aurélie, Kiwi John, Moreillon Philippe, Entenza José M., Bizzini Alain
ISSN
1098-6596 (Electronic)
ISSN-L
0066-4804
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
60
Number
9
Pages
5349-5356
Language
english
Abstract
In this study, silver/copper (Ag/Cu)-coated catheters were investigated for their efficacy in preventing methicillin-resistant Staphylococcus aureus (MRSA) infection in vitro and in vivoAg and Cu were sputtered (67/33% atomic ratio) on polyurethane catheters by Direct Current Magnetron Sputtering. In vitro, Ag/Cu-coated and uncoated catheters were immersed in PBS or rat plasma and exposed to 10(4)-10(8) CFU/ml of MRSA ATCC 43300. In vivo, Ag/Cu-coated and uncoated catheters were placed in the jugular vein of rats. Close by, MRSA (10(7) CFU/ml) was inoculated in the tail vein. Catheters were removed 48 h later and cultured.In vitro, Ag/Cu-coated catheters pre-incubated in PBS and exposed to 10(4)-10(7) CFU/ml, prevented the adherence of MRSA (0-12% colonization) compared to uncoated catheters (50-100% colonization; P< 0.005), Ag/Cu-coated catheters retained their activity (0-20% colonization) when pre-incubated in rat plasma while colonization of uncoated catheters increased (83-100%; P< 0.005). Ag/Cu-coating protection diminished with 10(8) CFU/ml in both PBS and plasma (50-100% colonization). In vivo, Ag/Cu-coated catheters reduced the incidence of catheter infection compared to uncoated catheters (57% vs 79%, respectively; P= 0.16) and bacteremia (31% vs 68%, respectively; P< 0.05). Scanning electron microscopy of explanted catheters suggests that the suboptimal activity of Ag/Cu catheters in vivo was due to the formation of a dense fibrin sheath over their surface.Ag/Cu-coated catheters have a potential for preventing MRSA infections. Their activity might be improved by limiting plasma protein adsorption on their surface.
Keywords
Catheters, Methicillin-Resistant Staphylococcus aureus, Silver, Copper
Pubmed
Web of science
Open Access
Yes
Create date
01/07/2016 10:16
Last modification date
20/08/2019 14:18
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