Validation of hepcidin quantification in plasma using LC-HRMS and discovery of a new hepcidin isoform.
Details
Serval ID
serval:BIB_313014089D6D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Validation of hepcidin quantification in plasma using LC-HRMS and discovery of a new hepcidin isoform.
Journal
Bioanalysis
ISSN
1757-6199 (Electronic)
ISSN-L
1757-6180
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
5
Number
20
Pages
2509-2520
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
BACKGROUND: Hepcidin, a 25 amino acid peptide, plays an important role in iron homeostasis. Some hepcidin truncated peptides have antibiotic effects.
RESULTS: A new analytical method for hepcidin determination in human plasma using LC-HRMS operating in full-scan acquisition mode has been validated. The extraction consists of protein precipitation and a drying reconstitution step; a 2.1 x 50 mm (idxL) C18 analytical column was used. Detection specificity, stability, accuracy, precision and recoveries were determined. The LOQ/LOD were 0.25/0.1 nM, respectively. More than 600 injections of plasma extracts were performed, allowing evaluation of the assay robustness. Hepcidin-20, hepcidin-22 and a new isoform, hepcidin-24, were detected in patients.
CONCLUSION: The data underscore the usefulness of LC-HRMS for in-depth investigations related to hepcidin levels and pathways.
RESULTS: A new analytical method for hepcidin determination in human plasma using LC-HRMS operating in full-scan acquisition mode has been validated. The extraction consists of protein precipitation and a drying reconstitution step; a 2.1 x 50 mm (idxL) C18 analytical column was used. Detection specificity, stability, accuracy, precision and recoveries were determined. The LOQ/LOD were 0.25/0.1 nM, respectively. More than 600 injections of plasma extracts were performed, allowing evaluation of the assay robustness. Hepcidin-20, hepcidin-22 and a new isoform, hepcidin-24, were detected in patients.
CONCLUSION: The data underscore the usefulness of LC-HRMS for in-depth investigations related to hepcidin levels and pathways.
Pubmed
Web of science
Create date
07/03/2014 19:02
Last modification date
20/08/2019 14:16