Validation of hepcidin quantification in plasma using LC-HRMS and discovery of a new hepcidin isoform.

Détails

ID Serval
serval:BIB_313014089D6D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Validation of hepcidin quantification in plasma using LC-HRMS and discovery of a new hepcidin isoform.
Périodique
Bioanalysis
Auteur(s)
Rochat B., Peduzzi D., McMullen J., Favre A., Kottelat E., Favrat B., Tissot J.D., Angelillo-Scherrer A., Bromirski M., Waldvogel S.
ISSN
1757-6199 (Electronic)
ISSN-L
1757-6180
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
5
Numéro
20
Pages
2509-2520
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
BACKGROUND: Hepcidin, a 25 amino acid peptide, plays an important role in iron homeostasis. Some hepcidin truncated peptides have antibiotic effects.
RESULTS: A new analytical method for hepcidin determination in human plasma using LC-HRMS operating in full-scan acquisition mode has been validated. The extraction consists of protein precipitation and a drying reconstitution step; a 2.1 x 50 mm (idxL) C18 analytical column was used. Detection specificity, stability, accuracy, precision and recoveries were determined. The LOQ/LOD were 0.25/0.1 nM, respectively. More than 600 injections of plasma extracts were performed, allowing evaluation of the assay robustness. Hepcidin-20, hepcidin-22 and a new isoform, hepcidin-24, were detected in patients.
CONCLUSION: The data underscore the usefulness of LC-HRMS for in-depth investigations related to hepcidin levels and pathways.
Pubmed
Web of science
Création de la notice
07/03/2014 18:02
Dernière modification de la notice
20/08/2019 13:16
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