24 Hours in the Life of HIV-1 in a T Cell Line.

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Serval ID
serval:BIB_2F7415BAC7BD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
24 Hours in the Life of HIV-1 in a T Cell Line.
Journal
Plos Pathogens
Author(s)
Mohammadi P., Desfarges S., Bartha I., Joos B., Zangger N., Muñoz M., Günthard H.F., Beerenwinkel N., Telenti A., Ciuffi A.
ISSN
1553-7374 (Electronic)
ISSN-L
1553-7366
Publication state
Published
Issued date
2013
Volume
9
Number
1
Pages
e1003161
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
HIV-1 infects CD4+ T cells and completes its replication cycle in approximately 24 hours. We employed repeated measurements in a standardized cell system and rigorous mathematical modeling to characterize the emergence of the viral replication intermediates and their impact on the cellular transcriptional response with high temporal resolution. We observed 7,991 (73%) of the 10,958 expressed genes to be modulated in concordance with key steps of viral replication. Fifty-two percent of the overall variability in the host transcriptome was explained by linear regression on the viral life cycle. This profound perturbation of cellular physiology was investigated in the light of several regulatory mechanisms, including transcription factors, miRNAs, host-pathogen interaction, and proviral integration. Key features were validated in primary CD4+ T cells, and with viral constructs using alternative entry strategies. We propose a model of early massive cellular shutdown and progressive upregulation of the cellular machinery to complete the viral life cycle.
Pubmed
Web of science
Open Access
Yes
Create date
13/02/2013 14:38
Last modification date
20/08/2019 13:13
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