Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes.
Details
Serval ID
serval:BIB_2BF9AC1F5402
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes.
Journal
Journal of Applied Bacteriology
ISSN
0021-8847 (Print)
ISSN-L
0021-8847
Publication state
Published
Issued date
1994
Peer-reviewed
Oui
Volume
77
Number
5
Pages
574-582
Language
english
Notes
Publication types: Comparative Study ; Journal Article Publication Status: ppublish
Abstract
The polymerase chain reaction was used to obtain randomly-amplified polymorphic DNA (RAPD) profiles from Listeria spp. and enterobacteria. Eleven different oligonucleotides were evaluated. Only one, HR4 (19mer), generated reproducible and specific profiles for Listeria spp., while results for enterobacteria were controversial. A total of 57 different Listeria strains were subjected to the RAPD analysis and 27 different profiles were recognized. RAPD typing allowed strains of the same serotype to be distinguished but the same profile was obtained from different serotypes of L. monocytogenes in three cases and in one case two different serotypes of L. innocua yielded the same profile. RAPD-typing with HR4 allowed L. monocytogenes contamination in several food outlets to be traced back to a food processing plant. In additional experiments, the general utility of this RAPD system in typing Yersinia enterocolitica, verotoxigenic Escherichia coli and Salmonella enteritidis was evaluated.
Keywords
Bacterial Typing Techniques, Base Sequence, DNA, Bacterial/genetics, DNA, Bacterial/isolation & purification, Enterobacteriaceae/classification, Enterobacteriaceae/genetics, Evaluation Studies as Topic, Food Contamination, Food Handling, Food Microbiology, Listeria monocytogenes/classification, Listeria monocytogenes/genetics, Meat Products/microbiology, Molecular Sequence Data, Oligonucleotides, Polymerase Chain Reaction/methods, Polymorphism, Genetic
Pubmed
Web of science
Create date
09/11/2014 15:44
Last modification date
20/08/2019 13:11
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