Perivascular Fibroblasts of the Developing Spleen Act as LTα1β2-Dependent Precursors of Both T and B Zone Organizer Cells.

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State: Public
Version: Final published version
Serval ID
serval:BIB_2BD5BF80996B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Perivascular Fibroblasts of the Developing Spleen Act as LTα1β2-Dependent Precursors of Both T and B Zone Organizer Cells.
Journal
Cell reports
Author(s)
Schaeuble K., Britschgi M.R., Scarpellino L., Favre S., Xu Y., Koroleva E., Lissandrin TKA, Link A., Matloubian M., Ware C.F., Nedospasov S.A., Tumanov A.V., Cyster J.G., Luther S.A.
ISSN
2211-1247 (Electronic)
Publication state
Published
Issued date
28/11/2017
Peer-reviewed
Oui
Volume
21
Number
9
Pages
2500-2514
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
T and B cell compartmentalization is a hallmark of secondary lymphoid organs and is maintained by chemokine-expressing stromal cells. How this stromal cell network initially develops and differentiates into two distinct subsets is poorly known, especially for the splenic white pulp (WP). Here, we show that perivascular fibroblast precursors are triggered by LTα1β2 signals to expand, express CCL19/21, and then differentiate into two functionally distinct fibroblast subsets responsible for B and T cell clustering and WP compartmentalization. Failure to express or sense CCL19 leads to impaired T zone development, while lack of B cells or LTα1β2 leads to an earlier and stronger impairment in WP development. We therefore propose that WP development proceeds in multiple steps, with LTα1β2 <sup>+</sup> B cells acting as major inducer cells driving the expansion and gradual differentiation of perivascular fibroblasts into T and B zone organizer cells.
Keywords
Animals, Cell Differentiation/physiology, Chemokine CCL19/metabolism, Chemokine CXCL13/metabolism, Chemokines, CXC/metabolism, Fibroblasts/metabolism, Fibroblasts/physiology, Lymphotoxin-alpha/metabolism, Mice, Spleen/cytology, Spleen/metabolism, CCR7, CXCL13, ILC3, LTi cells, PALS, chemokines, fibroblast heterogeneity, lymphocytes, lymphotoxin, stroma
Pubmed
Web of science
Open Access
Yes
Create date
09/12/2017 10:59
Last modification date
20/08/2019 14:11
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