Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections.

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Version: Author's accepted manuscript
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Serval ID
serval:BIB_2B9CC70589A8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections.
Journal
Laboratory investigation; a journal of technical methods and pathology
Author(s)
Brajkovic S., Dupouy D.G., de Leval L., Gijs M.A.
ISSN
1530-0307 (Electronic)
ISSN-L
0023-6837
Publication state
Published
Issued date
08/2017
Peer-reviewed
Oui
Volume
97
Number
8
Pages
983-991
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Frozen sections (FS) of tumor samples represent a cornerstone of pathological intraoperative consultation and have an important role in the microscopic analysis of specimens during surgery. So far, immunohistochemical (IHC) stainings on FS have been demonstrated for a few markers using manual methods. Microfluidic technologies have proven to bring substantial improvement in many fields of diagnostics, though only a few microfluidic devices have been designed to improve the performance of IHC assays. In this work, we show optimization of a complete pan-cytokeratin chromogenic immunostaining protocol on FS using a microfluidic tissue processor into a protocol taking <12 min. Our results showed specificity and low levels of background. The dimensions of the microfluidic prototype device are compatible with the space constraints of an intraoperative pathology laboratory. We therefore anticipate that the adoption of microfluidic technologies in the field of surgical pathology can significantly improve the way FSs influence surgical procedures.

Keywords
Breast/diagnostic imaging, Coloring Agents/chemistry, Equipment Design, Female, Humans, Immunohistochemistry/instrumentation, Immunohistochemistry/methods, Keratins/analysis, Keratins/chemistry, Keratins/metabolism, Male, Microfluidic Analytical Techniques/instrumentation, Microfluidic Analytical Techniques/methods, Neoplasms/diagnostic imaging, Prostate/diagnostic imaging, Ureter/diagnostic imaging
Pubmed
Web of science
Open Access
Yes
Create date
31/05/2017 12:08
Last modification date
20/08/2019 13:11
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