Diurnal regulation of RNA polymerase III transcription is under the control of both the feeding-fasting response and the circadian clock.

Details

Ressource 1Download: Genome Res.-2017-Mange-973-84.pdf (1488.56 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_240C54C8CA8A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Diurnal regulation of RNA polymerase III transcription is under the control of both the feeding-fasting response and the circadian clock.
Journal
Genome research
Author(s)
Mange F., Praz V., Migliavacca E., Willis I.M., Schütz F., Hernandez N.
Working group(s)
CycliX Consortium
Contributor(s)
Hernandez N., Delorenzi M., Deplancke B., Desvergne B., Guex N., Herr W., Naef F., Rougemont J., Schibler U., Andersin T., Cousin P., Gilardi F., Lammers F., Mange F., Villeneuve D., David F., Fabbretti R., Jacquet P., Krier I., Kuznetsov D., Leleu M., Liechti R., Martin O., Migliavacca E., Naldi A., Praz V., Rib L., Sobel J., Vlegel V., Xenarios I.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Publication state
Published
Issued date
06/2017
Peer-reviewed
Oui
Volume
27
Number
6
Pages
973-984
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
RNA polymerase III (Pol III) synthesizes short noncoding RNAs, many of which are essential for translation. Accordingly, Pol III activity is tightly regulated with cell growth and proliferation by factors such as MYC, RB1, TRP53, and MAF1. MAF1 is a repressor of Pol III transcription whose activity is controlled by phosphorylation; in particular, it is inactivated through phosphorylation by the TORC1 kinase complex, a sensor of nutrient availability. Pol III regulation is thus sensitive to environmental cues, yet a diurnal profile of Pol III transcription activity is so far lacking. Here, we first use gene expression arrays to measure mRNA accumulation during the diurnal cycle in the livers of (1) wild-type mice, (2) arrhythmic javax.xml.bind.JAXBElement@59c2c50e knockout mice, (3) mice fed at regular intervals during both night and day, and (4) mice lacking the javax.xml.bind.JAXBElement@160cb27a gene, and so provide a comprehensive view of the changes in cyclic mRNA accumulation occurring in these different systems. We then show that Pol III occupancy of its target genes rises before the onset of the night, stays high during the night, when mice normally ingest food and when translation is known to be increased, and decreases in daytime. Whereas higher Pol III occupancy during the night reflects a MAF1-dependent response to feeding, the rise of Pol III occupancy before the onset of the night reflects a circadian clock-dependent response. Thus, Pol III transcription during the diurnal cycle is regulated both in response to nutrients and by the circadian clock, which allows anticipatory Pol III transcription.

Keywords
ARNTL Transcription Factors/deficiency, ARNTL Transcription Factors/genetics, Animals, Circadian Clocks/genetics, Circadian Rhythm/genetics, Eating/genetics, Fasting/metabolism, Gene Expression Profiling, Gene Expression Regulation, Liver/metabolism, Mice, Mice, Knockout, Oligonucleotide Array Sequence Analysis, Protein Binding, RNA Polymerase III/genetics, RNA Polymerase III/metabolism, Repressor Proteins/deficiency, Repressor Proteins/genetics, Signal Transduction, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
04/04/2017 19:09
Last modification date
19/02/2020 11:56
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