Coactivation of AP-1 activity and TGF-beta1 gene expression in the stress response of normal skin cells to ionizing radiation.

Details

Serval ID
serval:BIB_231B6FD88EBC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Coactivation of AP-1 activity and TGF-beta1 gene expression in the stress response of normal skin cells to ionizing radiation.
Journal
Oncogene
Author(s)
Martin M., Vozenin M.C., Gault N., Crechet F., Pfarr C.M., Lefaix J.L.
ISSN
0950-9232 (Print)
ISSN-L
0950-9232
Publication state
Published
Issued date
18/08/1997
Peer-reviewed
Oui
Volume
15
Number
8
Pages
981-989
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Activation of the AP-1 transcription factor and TGF-beta1 growth factor by ionizing radiation was studied both in vivo in pig skin, and in vitro in human fibroblasts and keratinocytes. Three and 6 h after irradiation, the Fos and Jun proteins and their binding activity to an AP-1 consensus sequence were strongly induced by high doses of gamma-rays. c-Fos, c-Jun and JunB proteins were found to be present in gel-shift complexes by probing with specific antibodies. Both keratinocytes and fibroblasts exhibited heightened AP-1 activity following irradiation. As we previously found that TGF-beta1 is involved in the development of skin lesions induced by radiation, TGF-beta1 gene expression was also examined. Two and 6 h after irradiation, the levels of TGF-beta1 transcripts were increased in skin. By immunostaining, TGF-beta1 protein levels were found to be increased in fibroblasts, keratinocytes and endothelial cells. As the TGF-beta1 promoter contains AP-1 binding sites, the relation between AP-1 activity and TGF-beta1 induction was addressed. The -365 TGF-beta1 promoter fragment, which contains a high affinity AP-1 site, exhibited increased binding to Jun and Fos proteins following irradiation. These results suggest that stress-inducible TGF-beta1 expression is mediated by the activation of AP-1 transcription factor.

Keywords
Animals, Cells, Cultured, Consensus Sequence, Dose-Response Relationship, Radiation, Gene Expression/radiation effects, Humans, Oxidative Stress, Promoter Regions, Genetic, Skin/metabolism, Skin/radiation effects, Swine, Transcription Factor AP-1/metabolism, Transforming Growth Factor beta/biosynthesis, Transforming Growth Factor beta/genetics
Pubmed
Web of science
Open Access
Yes
Create date
27/04/2018 15:02
Last modification date
20/08/2019 13:00
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