Coactivation of AP-1 activity and TGF-beta1 gene expression in the stress response of normal skin cells to ionizing radiation.
Details
Serval ID
serval:BIB_231B6FD88EBC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Coactivation of AP-1 activity and TGF-beta1 gene expression in the stress response of normal skin cells to ionizing radiation.
Journal
Oncogene
ISSN
0950-9232 (Print)
ISSN-L
0950-9232
Publication state
Published
Issued date
18/08/1997
Peer-reviewed
Oui
Volume
15
Number
8
Pages
981-989
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Activation of the AP-1 transcription factor and TGF-beta1 growth factor by ionizing radiation was studied both in vivo in pig skin, and in vitro in human fibroblasts and keratinocytes. Three and 6 h after irradiation, the Fos and Jun proteins and their binding activity to an AP-1 consensus sequence were strongly induced by high doses of gamma-rays. c-Fos, c-Jun and JunB proteins were found to be present in gel-shift complexes by probing with specific antibodies. Both keratinocytes and fibroblasts exhibited heightened AP-1 activity following irradiation. As we previously found that TGF-beta1 is involved in the development of skin lesions induced by radiation, TGF-beta1 gene expression was also examined. Two and 6 h after irradiation, the levels of TGF-beta1 transcripts were increased in skin. By immunostaining, TGF-beta1 protein levels were found to be increased in fibroblasts, keratinocytes and endothelial cells. As the TGF-beta1 promoter contains AP-1 binding sites, the relation between AP-1 activity and TGF-beta1 induction was addressed. The -365 TGF-beta1 promoter fragment, which contains a high affinity AP-1 site, exhibited increased binding to Jun and Fos proteins following irradiation. These results suggest that stress-inducible TGF-beta1 expression is mediated by the activation of AP-1 transcription factor.
Keywords
Animals, Cells, Cultured, Consensus Sequence, Dose-Response Relationship, Radiation, Gene Expression/radiation effects, Humans, Oxidative Stress, Promoter Regions, Genetic, Skin/metabolism, Skin/radiation effects, Swine, Transcription Factor AP-1/metabolism, Transforming Growth Factor beta/biosynthesis, Transforming Growth Factor beta/genetics
Pubmed
Web of science
Open Access
Yes
Create date
27/04/2018 16:02
Last modification date
20/08/2019 14:00