Effective gene expression in Pseudomonas aeruginosa under the control of the Escherichia coli consensus promoter

Details

Serval ID
serval:BIB_2266222E78D7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Effective gene expression in Pseudomonas aeruginosa under the control of the Escherichia coli consensus promoter
Journal
FEMS Microbiology Letters
Author(s)
Soldati L., Jeenes D. J., Haas D.
ISSN
0378-1097
Publication state
Published
Issued date
1987
Volume
42
Number
2-3
Pages
163-167
Language
english
Abstract
Promoter probe fragments containing either the Escherichia coli lacZ (β-galactosidase) gene or the Pseudomonas aeruginosa proC (Δ1-pyrroline 5-carboxylate reductase) gene were fused to the E. coli consensus (tac) promoter and cloned into the broad-host-range vector plasmid pKT240, which also carried the lacIQ repressor gene. Upon induction with isopropyl β-d-thiogalactoside similarly high lacZ and proC enzyme levels were obtained in both E. coli and P. aeruginosa. The E. coli argF promoter, which deviates from the consensus sequence, was clearly less efficient in both hosts. We conclude that the tac/lacIQ system functions well in P. aeruginosa and that no major expression barrier exists between E. coli and P. aeruginosa at the translational level.
Keywords
Pseudomonas aeruginosa, Gene expression, Escherichia coli, Consensus promoter, Beta galactosidase, Δ1-pyrroline 5-carboxylate reductase
Web of science
Open Access
Yes
Create date
04/03/2008 17:24
Last modification date
20/08/2019 12:59
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