Effective gene expression in Pseudomonas aeruginosa under the control of the Escherichia coli consensus promoter

Détails

ID Serval
serval:BIB_2266222E78D7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Effective gene expression in Pseudomonas aeruginosa under the control of the Escherichia coli consensus promoter
Périodique
FEMS Microbiology Letters
Auteur(s)
Soldati L., Jeenes D. J., Haas D.
ISSN
0378-1097
Statut éditorial
Publié
Date de publication
1987
Volume
42
Numéro
2-3
Pages
163-167
Langue
anglais
Résumé
Promoter probe fragments containing either the Escherichia coli lacZ (β-galactosidase) gene or the Pseudomonas aeruginosa proC (Δ1-pyrroline 5-carboxylate reductase) gene were fused to the E. coli consensus (tac) promoter and cloned into the broad-host-range vector plasmid pKT240, which also carried the lacIQ repressor gene. Upon induction with isopropyl β-d-thiogalactoside similarly high lacZ and proC enzyme levels were obtained in both E. coli and P. aeruginosa. The E. coli argF promoter, which deviates from the consensus sequence, was clearly less efficient in both hosts. We conclude that the tac/lacIQ system functions well in P. aeruginosa and that no major expression barrier exists between E. coli and P. aeruginosa at the translational level.
Mots-clé
Pseudomonas aeruginosa, Gene expression, Escherichia coli, Consensus promoter, Beta galactosidase, Δ1-pyrroline 5-carboxylate reductase
Web of science
Open Access
Oui
Création de la notice
04/03/2008 17:24
Dernière modification de la notice
20/08/2019 12:59
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