T cell receptor specificity is critical for the development of epidermal gammadelta T cells.
Details
State: Public
Version: author
Serval ID
serval:BIB_20717
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
T cell receptor specificity is critical for the development of epidermal gammadelta T cells.
Journal
The Journal of Experimental Medicine
ISSN
0022-1007
Publication state
Published
Issued date
2001
Peer-reviewed
Oui
Volume
194
Number
10
Pages
1473-1483
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
A particular feature of gammadelta T cell biology is that cells expressing T cell receptor (TCR) using specific Vgamma/Vdelta segments are localized in distinct epithelial sites, e.g., in mouse epidermis nearly all gammadelta T cells express Vgamma3/Vdelta1. These cells, referred to as dendritic epidermal T cells (DETC) originate from fetal Vgamma3+ thymocytes. The role of gammadelta TCR specificity in DETC's migration/localization to the skin has remained controversial. To address this issue we have generated transgenic (Tg) mice expressing a TCR delta chain (Vdelta6.3-Ddelta1-Ddelta2-Jdelta1-Cdelta), which can pair with Vgamma3 in fetal thymocytes but is not normally expressed by DETC. In wild-type (wt) Vdelta6.3Tg mice DETC were present and virtually all of them express Vdelta6.3. However, DETC were absent in TCR-delta(-/-) Vdelta6.3Tg mice, despite the fact that Vdelta6.3Tg gammadelta T cells were present in normal numbers in other lymphoid and nonlymphoid tissues. In wt Vdelta6.3Tg mice, a high proportion of in-frame Vdelta1 transcripts were found in DETC, suggesting that the expression of an endogenous TCR-delta (most probably Vdelta1) was required for the development of Vdelta6.3+ epidermal gammadelta T cells. Collectively our data demonstrate that TCR specificity is essential for the development of gammadelta T cells in the epidermis. Moreover, they show that the TCR-delta locus is not allelically excluded.
Keywords
Animals, Cell Movement, Dendritic Cells/physiology, Mice, Mice, Inbred C57BL, Receptors, Antigen, T-Cell, gamma-delta/genetics, Receptors, Antigen, T-Cell, gamma-delta/physiology, Skin/immunology, Stem Cells/physiology, T-Lymphocytes/physiology, Thymus Gland/cytology
OAI-PMH
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 13:15
Last modification date
20/08/2019 13:56