Role of phosphoinositide 3-kinase in the autophagic death of serum-deprived PC12 cells.

Details

Serval ID
serval:BIB_1EF1B6863FBF
Type
Article: article from journal or magazin.
Collection
Publications
Title
Role of phosphoinositide 3-kinase in the autophagic death of serum-deprived PC12 cells.
Journal
Apoptosis : an International Journal on Programmed Cell Death
Author(s)
Guillon-Munos A., van Bemmelen M.X., Clarke P.G.
ISSN
1360-8185 (Print)
ISSN-L
1360-8185
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
10
Number
5
Pages
1031-1041
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The death of serum-deprived undifferentiated PC12 cells shows both autophagic and apoptotic features. Since it is still controversial whether the autophagy is instrumental in the cell death or a mere epiphenomenon, we tested the effects of inhibiting the autophagy by a variety of phosphoinositide 3-kinase inhibitors, and provided evidence that the autophagy, or a related trafficking event, is indeed instrumental in the cell death. Furthermore, by comparing the effects of PI3-K inhibition and caspase-inhibition on autophagic and apoptotic cellular events, we showed that in this case the autophagic and apoptotic mechanisms mediate cell death by parallel pathways and do not act in series.
Keywords
Acid Phosphatase/analysis, Animals, Apoptosis/drug effects, Apoptosis/physiology, Autophagy/drug effects, Autophagy/physiology, Caspase Inhibitors, Chromones/pharmacology, Culture Media, Serum-Free/pharmacology, Immunohistochemistry, Lysosomes/physiology, Microscopy, Electron, Morpholines/pharmacology, PC12 Cells/cytology, Phosphatidylinositol 3-Kinases/antagonists & inhibitors, Phosphatidylinositol 3-Kinases/biosynthesis, Rats
Pubmed
Web of science
Create date
20/01/2008 18:49
Last modification date
03/03/2018 14:36
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