Rheumatoid factor autoantibody-binding site: a molecular analysis using monoclonal antibodies with dual anti-TNP and anti-IgG activities
Details
Serval ID
serval:BIB_1D0CDE3835A5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rheumatoid factor autoantibody-binding site: a molecular analysis using monoclonal antibodies with dual anti-TNP and anti-IgG activities
Journal
European Journal of Immunology
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
11/1989
Volume
19
Number
11
Pages
2123-30
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
Two out of five murine IgG3 anti-trinitrophenyl (TNP) monoclonal antibodies (mAb) obtained either by immunization with TNP-keyhole limpet hemocyanin (KLH) (CB1, CB5, CB6 and 4H10) or with dinitrophenyl-lipopolysaccharide (9A6), exhibited anti-IgG rheumatoid factor (RF) activity (CB6 and 4H10). The anti-IgG activity of these two anti-TNP RF was specifically inhibited by murine IgG as well as by the hapten TNP. In order to identify the structural basis for the anti-IgG activity, the nucleotide sequences encoding the VH and VL regions were determined. By comparing the V regions of the non-RF and RF anti-TNP mAb, it was found that one anti-TNP RF antibody, CB6, shares virtually identical VL and VH regions with two anti-TNP antibodies, CB1 and CB5, but markedly differs from these in the D region. Furthermore, the light chain framework region 2 (FR2) and FR3 of non-RF mAb, CB1, CB5 and 9A6, have amino acid sequences almost identical to those claimed for anti-IgG1 RF activity (Shlomchik et al., J. Exp. Med. 1986. 164: 407). Our findings suggest, at least in the case of CB6 mAb, the involvement of CDR, but not light chain FR residues, in IgG binding.
Keywords
Amino Acid Sequence
Animals
Antibodies, Monoclonal/immunology
Autoantibodies/genetics/*immunology
Base Sequence
Binding Sites, Antibody
Immunoglobulin Heavy Chains/physiology
Immunoglobulin Light Chains/physiology
Immunoglobulin Variable Region/*genetics/physiology
Mice
Mice, Inbred Strains
Molecular Sequence Data
RNA, Messenger/genetics
Rheumatoid Factor/genetics/*immunology
Trinitrobenzenes/immunology
Pubmed
Web of science
Create date
25/01/2008 15:20
Last modification date
20/08/2019 12:53