Local Pheromone Release from Dynamic Polarity Sites Underlies Cell-Cell Pairing during Yeast Mating.

Details

Ressource 1Download: Merlini2016.pdf (5368.75 [Ko])
State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_1BAD3FBF32E6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Local Pheromone Release from Dynamic Polarity Sites Underlies Cell-Cell Pairing during Yeast Mating.
Journal
Current biology
Author(s)
Merlini L., Khalili B., Bendezú F.O., Hurwitz D., Vincenzetti V., Vavylonis D., Martin S.G.
ISSN
1879-0445 (Electronic)
ISSN-L
0960-9822
Publication state
Published
Issued date
25/04/2016
Peer-reviewed
Oui
Volume
26
Number
8
Pages
1117-1125
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Cell pairing is central for many processes, including immune defense, neuronal connection, hyphal fusion, and sexual reproduction. How does a cell orient toward a partner, especially when faced with multiple choices? Fission yeast Schizosaccharomyces pombe P and M cells, which respectively express P and M factor pheromones [1, 2], pair during the mating process induced by nitrogen starvation. Engagement of pheromone receptors Map3 and Mam2 [3, 4] with their cognate pheromone ligands leads to activation of the Gα protein Gpa1 to signal sexual differentiation [3, 5, 6]. Prior to cell pairing, the Cdc42 GTPase, a central regulator of cell polarization, forms dynamic zones of activity at the cell periphery at distinct locations over time [7]. Here we show that Cdc42-GTP polarization sites contain the M factor transporter Mam1, the general secretion machinery, which underlies P factor secretion, and Gpa1, suggesting that these are sub-cellular zones of pheromone secretion and signaling. Zone lifetimes scale with pheromone concentration. Computational simulations of pair formation through a fluctuating zone show that the combination of local pheromone release and sensing, short pheromone decay length, and pheromone-dependent zone stabilization leads to efficient pair formation. Consistently, pairing efficiency is reduced in the absence of the P factor protease. Similarly, zone stabilization at reduced pheromone levels, which occurs in the absence of the predicted GTPase-activating protein for Ras, leads to reduction in pairing efficiency. We propose that efficient cell pairing relies on fluctuating local signal emission and perception, which become locked into place through stimulation.

Keywords
ATP-Binding Cassette Transporters/metabolism, Cell Fusion, Cell Polarity, Pheromones/metabolism, Schizosaccharomyces/cytology, Schizosaccharomyces/physiology, Schizosaccharomyces pombe Proteins/chemistry, Schizosaccharomyces pombe Proteins/metabolism, Signal Transduction, cdc42 GTP-Binding Protein/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
27/05/2016 9:36
Last modification date
20/08/2019 12:52
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