DNA degradation in avian faecal samples and feasibility of non-invasive genetic studies applied to threatened capercaillie populations

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Serval ID
serval:BIB_1ABC56DED65D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
DNA degradation in avian faecal samples and feasibility of non-invasive genetic studies applied to threatened capercaillie populations
Journal
Conservation Genetics
Author(s)
Regnaut  S., Lucas  F. S., Fumagalli  L.
ISSN
1566-0621
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
7
Number
3
Pages
449-453
Language
english
Abstract
We evaluated the feasibility of using faeces as a non-invasively collected DNA source for the genetic study of an endangered bird population (capercaillie; Tetrao urogallus). We used a multitube approach, and for our panel of 11 microsatellites genotyping reliability was estimated at 98% with five repetitions. Experiments showed that free DNases in faecal material were the major cause of DNA degradation. Our results demonstrate that using avian faeces as a source of DNA, reliable microsatellite genotyping can be obtained with a reasonable number of PCR replicates.
Keywords
bird, DNA degradation, faeces, genotyping errors, microsatellites, non-invasive sampling
Web of science
Open Access
Yes
Create date
24/01/2008 20:09
Last modification date
01/10/2019 7:17
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