Investigation of the hepatitis C virus replication complex.

Details

Serval ID
serval:BIB_1A72E12DC17D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Investigation of the hepatitis C virus replication complex.
Journal
Methods in Molecular Biology
Author(s)
Brass V., Gosert R., Moradpour D.
ISSN
1064-3745
Publication state
Published
Issued date
2009
Volume
510
Pages
195-209
Language
english
Abstract
Formation of a membrane-associated replication complex, composed of viral proteins, replicating RNA, altered cellular membranes, and other host factors, is a hallmark of all positive-strand RNA viruses. In the case of HCV, RNA replication takes place in a likely endoplasmic reticulum-derived membrane alteration referred to as the "membranous web." In vitro transcription-translation, membrane extraction and flotation analyses, immunofluorescence microscopy, fluorescent in situ hybridization, and RNA metabolic labeling followed by confocal laser scanning microscopy have yielded insights into the structure and function of the HCV replication complex. We describe these techniques and highlight selected results.
Keywords
Animals, Bromodeoxyuridine/metabolism, Cell Membrane/metabolism, Fluorescent Antibody Technique, Hepacivirus/metabolism, In Situ Hybridization, Fluorescence, Protein Biosynthesis, RNA, Viral/biosynthesis, Staining and Labeling, Transcription, Genetic, Viral Proteins/biosynthesis, Viral Proteins/chemistry, Virus Replication
Pubmed
Create date
11/09/2009 8:28
Last modification date
20/08/2019 12:51
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